Nucleotide Phosphohydrolase in Purified Vaccinia Virus
نویسندگان
چکیده
منابع مشابه
Deoxyribonucleic acid-dependent nucleotide phosphohydrolase activity in purified vaccinia virus.
A nucleotide phosphohydrolase (adenosine triphosphatase), which is associated with vaccinia virus cores, has been solubilized and shown to be deoxyribonucleic acid dependent.
متن کاملCharacteristics of reactions catalyzed by purified guanylyltransferase from vaccinia virus.
GTP:RNA guanylyltransferase catalyzes the initial reaction in the formation of “caps” at 5’ termini of mRNAs. The characteristics of the guanylation reaction have been studied using purified enzyme isolated from vaccinia virus. Guanylation requires a divalent cation (Mg2’), is stimulated by monovalent cations (Na+ and K+), and is unaffected by S-adenosylmethionine. The enzyme utilizes GTP as gu...
متن کاملMutational analysis of vaccinia virus nucleoside triphosphate phosphohydrolase II, a DExH box RNA helicase.
Vaccinia virus nucleoside triphosphate phosphohydrolase II (NPH-II), a 3'-to-5' RNA helicase, displays sequence similarity to members of the DExH family of nucleic acid-dependent nucleoside triphosphatases (NTPases). The contributions of the conserved GxGKT and DExH motifs to enzyme activity were assessed by alanine scanning mutagenesis. Histidine-tagged versions of NPH-II were expressed in vac...
متن کاملNTP binding and phosphohydrolase activity associated with purified bluetongue virus non-structural protein NS2.
The bluetongue virus ssRNA-binding protein, NS2, is a phosphoprotein that forms viral inclusion bodies in infected cells. Recombinant NS2 was expressed in the baculovirus expression system and purified to homogeneity from insect cells. Purified NS2 bound nucleosides. Further investigation revealed that the protein bound ATP and GTP and could hydrolyse both nucleosides to their corresponding NMP...
متن کاملEctophosphodiesterase/nucleotide phosphohydrolase (Enpp) nucleotidases: cloning, conservation and developmental restriction.
Ectonucleotidase proteins occupy a central role in purine signalling regulation by sequentially hydrolysing ATP to ADP and to adenosine. The ENPP ( or PDNP) gene family, which encodes ectophosphodiesterase/nucleotide phosphohydrolases, is a subfamily of these enzymes, which consists of 7 members in mammals. These proteins catalyse the generation of bioactive lipids, placing the ENPP enzymes as ...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1968
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.2.3.167-172.1968